Article
Crystal structure of the cataract-causing P23T γD-crystallin mutant.
Proteins - 1 Sept 2013
Ji Fangling, Koharudin Leonardus M I, Jung Jinwon, Gronenborn Angela M
Abstract excerpt
Up to now, efforts to crystallize the cataract-associated P23T mutant of human γD-crystallin have not been successful. Therefore, insights into the light scattering mechanism of this mutant have been exclusively obtained from solution work. Here we present the first crystal structure of the P23T mutant at 2.5 Å resolution. The protein exhibits essentially the same overall structure as seen for the wild-type...
Topics
- Cataract
- Escherichia coli
- Humans
- Models, Molecular
- Mutation
- Recombinant Proteins
- gamma-Crystallins