Article
[Construction and identification of a recombinant PRRSV expressing protective antigens of type O foot-and-mouth disease virus].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Dec 2012
Tong Wu, Xu Yanzhao, Zhou Yanjun, Jiang Yifeng, Zhang Shanrui, Wang Yaxin, Zhu Jianping, Yu Lingxue, Sun Jing, Chen Huanchun, Tong Guangzhi
Abstract excerpt
Using mutation PCR, we cloned the target gene containing 421-480nt (141-160aa) and 598-639nt (200-213aa) of VP1 gene of foot and mouth disease virus (FMDV) into the deleted region (508-532aa) of Nsp2 gene of a highly pathogenic porcine reproductive and respiratory syndrome virus derived vaccine strain (HuN4-F112) that was used as vector. The recombinant cDNA was in vitro transcribed followed by transfection of...
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