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[Recovery of an infectious virus from the full-length cDNA of PRRSV BJ-4].

Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Jun 2007

Ran Zhi-guang, Chen Xiao-yun, Yang Han-chun, Guo Xin, Gai Xin-na

Abstract excerpt

Six recombinant plasmids covering cDNA of porcine reproductive and respiratory syndrome virus BJ-4 were sequenced, respectively, and 23 point mutations were reverted with site-directed mutagenesis kit. The full-length cDNA clone pWSK-DCBA was assembled and re-sequenced. The capped viral genomic RNA was transcribed in vitro, mixed with liposome and transfected into MARC-145 cells, and an infectious virus...

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PMID: 17672299

Genes

CPE

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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