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Article

Monomeric TonB and the Ton box are required for the formation of a high-affinity transporter-TonB complex.

Biochemistry - 16 Apr 2013

Freed Daniel M, Lukasik Stephen M, Sikora Arthur, Mokdad Audrey, Cafiso David S

Abstract excerpt

The energy-dependent uptake of trace nutrients by Gram-negative bacteria involves the coupling of an outer membrane transport protein to the transperiplasmic protein TonB. In this study, a soluble construct of Escherichia coli TonB (residues 33-239) was used to determine the affinity of TonB for outer membrane transporters BtuB, FecA, and FhuA. Using fluorescence anisotropy, TonB(33-239) was found to bind with...

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Identifiers

PMID: 23517233
DOI: 10.1021/bi3016108
PMCID: PMC3630801

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Monomeric TonB and the Ton box are required for the formation of a high-affinity transporter-TonB complex.PMID 23517233Read ↗

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TonB and the gram-negative dilemma.Semantic proximity Read ↗

TonB system, in vivo assays and characterization.Semantic proximity Read ↗

Regions of Escherichia coli TonB and FepA proteins essential for in vivo physical interactions.Semantic proximity Read ↗

Functional Diversity of TonB-Like Proteins in the Heterocyst-Forming Cyanobacterium Anabaena sp. PCC 7120.Semantic proximity Read ↗

Evidence of Fe3+ interaction with the plug domain of the outer membrane transferrin receptor protein of Neisseria gonorrhoeae: implications for Fe transport.Semantic proximity Read ↗

The plug domain of a neisserial TonB-dependent transporter retains structural integrity in the absence of its transmembrane beta-barrel.Semantic proximity Read ↗

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Energy transduction between membranes. TonB, a cytoplasmic membrane protein, can be chemically cross-linked in vivo to the outer membrane receptor FepA.Semantic proximity Read ↗

Substrate-induced exposure of an energy-coupling motif of a membrane transporter.Semantic proximity Read ↗

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