Article
A transgene expression system for the marine microalgae Aurantiochytrium sp. KRS101 using a mutant allele of the gene encoding ribosomal protein L44 as a selectable transformation marker for cycloheximide resistance.
Bioprocess and biosystems engineering - 1 Sept 2013
Hong Won-Kyung, Heo Sun-Yeon, Oh Baek-Rock, Kim Chul Ho, Sohn Jung-Hoon, Yang Ji-Won, Kondo Akihiko, Seo Jeong-Woo
Abstract excerpt
In the present study, we established a genetic system for manipulating the oleaginous heterotrophic microalgae Aurantiochytrium sp. KRS101, using cycloheximide resistance as the selectable marker. The gene encoding ribosomal protein L44 (RPL44) of Aurantiochytrium sp. KRS101 was first identified and characterized. Proline 56 was replaced with glutamine, affording cycloheximide resistance to strains encoding the...
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