Rapid, economical single-nucleotide polymorphism and microsatellite discovery based on de novo assembly of a reduced representation genome in a non-model organism: a case study of Atlantic cod Gadus morhua.

Abstract excerpt

By combining next-generation sequencing technology (454) and reduced representation library (RRL) construction, the rapid and economical isolation of over 25 000 potential single-nucleotide polymorphisms (SNP) and >6000 putative microsatellite loci from c. 2% of the genome of the non-model teleost, Atlantic cod Gadus morhua from the Celtic Sea, south of Ireland, was demonstrated. A small-scale validation of...

Topics

• Animals
• Feasibility Studies
• Gadus morhua
• Genetics, Population
• Genomics
• Genotyping Techniques
• Ireland
• Microsatellite Repeats
• Oceans and Seas
• Polymorphism, Single Nucleotide