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Single nucleotide polymorphism discovery in rainbow trout by deep sequencing of a reduced representation library.

BMC genomics - 25 Nov 2009

Sánchez Cecilia Castaño, Smith Timothy P L, Wiedmann Ralph T, Vallejo Roger L, Salem Mohamed, Yao Jianbo, Rexroad Caird E

Abstract excerpt

BACKGROUND: To enhance capabilities for genomic analyses in rainbow trout, such as genomic selection, a large suite of polymorphic markers that are amenable to high-throughput genotyping protocols must be identified. Expressed Sequence Tags (ESTs) have been used for single nucleotide polymorphism (SNP) discovery in salmonids. In those strategies, the salmonid semi-tetraploid genomes often led to assemblies of...

Topics

Animals
DNA
Female
Genomic Library
High-Throughput Screening Assays
Male
Oncorhynchus mykiss
Polymorphism, Single Nucleotide
Reproducibility of Results
Sequence Analysis, DNA

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PMID: 19939274
DOI: 10.1186/1471-2164-10-559
PMCID: PMC2790473

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