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Polymerase chain reaction for detection of Toxoplasma gondii

1 May 1990

Abstract excerpt

A DNA-based assay has been developed for the detection of Toxoplasma gondii. The assay makes use of the polymerase chain reaction (PCR) to amplify part of the P30 gene on the parasite's DNA. Following gel electrophoresis, the amplified DNA can be detected either directly on the gel or by Southern hybridisation with radioactive or non-radioactive DNA probes. The assay has been used to detect the DNA from different...

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PMID: 2342084
DOI: 10.1099/00222615-32-1-25

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