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Real-Time PCR for Quantitative Detection of Toxoplasma gondii

1 Nov 2000

Abstract excerpt

The protozoan Toxoplasma gondii is one of the most common infectious pathogenic parasites and can cause severe medical complications in infants and immunocompromised individuals. We report here the development of a real-time PCR-based assay for the detection of T. gondii. Oligonucleotide primers and a fluorescence-labeled TaqMan probe were designed to amplify the T. gondii B1 gene. After 40 PCR cycles, the cycle...

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PMID: 11060078
DOI: 10.1128/jcm.38.11.4121-4125.2000

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