Comparison of two gene amplification methods for the detection of Toxoplasma gondii in experimentally infected sheep

Abstract excerpt

Efferent lymph and peripheral blood collected from sheep experimentally infected with Toxoplasma gondii strain S48 were analysed for parasite DNA by amplification of the B1 and P30 T. gondii genes by the polymerase chain reaction (PCR). The relative sensitivity of these two gene amplification methods was assessed and compared with parasite detection by mouse injection (MI). B1 PCR was consistently more sensitive...

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