Article
Development of a colon cancer GEMM-derived orthotopic transplant model for drug discovery and validation.
Clinical cancer research : an official journal of the American Association for Cancer Research - 1 Jun 2013
Martin Eric S, Belmont Peter J, Sinnamon Mark J, Richard Larissa Georgeon, Yuan Jing, Coffee Erin M, Roper Jatin, Lee Lydia, Heidari Pedram, Lunt Sophia Y, Goel Gautam, Ji Xiadong, Xie Zhi, Xie Tao, Lamb John, Weinrich Scott L, VanArsdale Todd, Bronson Roderick T, Xavier Ramnik J, Vander Heiden Matthew G, Kan Julie L C, Mahmood Umar, Hung Kenneth E
Abstract excerpt
PURPOSE: Effective therapies for KRAS-mutant colorectal cancer (CRC) are a critical unmet clinical need. Previously, we described genetically engineered mouse models (GEMM) for sporadic Kras-mutant and non-mutant CRC suitable for preclinical evaluation of experimental therapeutics. To accelerate drug discovery and validation, we sought to derive low-passage cell lines from GEMM Kras-mutant and wild-type tumors...
Topics
- Animals
- Carcinogenesis
- Cell Line, Tumor
- Cluster Analysis
- Colonic Neoplasms
- Disease Models, Animal
- Gene Expression Profiling
- Genes, APC