Article
Development of a method to quantitate nematode pheromone for study of small-molecule metabolism in Caenorhabditis elegans.
Analytical chemistry - 5 Mar 2013
Kim Kwang-Youl, Joo Hyoe-Jin, Kwon Hye-Won, Kim Heekyeong, Hancock William S, Paik Young-Ki
Abstract excerpt
Pheromones produced by Caenorhabditis elegans are considered key regulators of development, mating, and social behaviors in this organism. Here, we present a rapid mass spectrometry-based method (PheroQu) for absolute quantitation of nematode pheromones (e.g., daumone 1, 2, and 3) both in C. elegans worm bodies (as few as 20 worms) and in liquid culture medium. Pheromones were separated by ultra performance...
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