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Article

Catalysis by N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase (PIG-L) from Entamoeba histolytica: new roles for conserved residues.

The Journal of biological chemistry - 15 Mar 2013

Ashraf Mohammad, Sreejith Perinthottathil, Yadav Usha, Komath Sneha Sudha

Abstract excerpt

We showed previously that Entamoeba histolytica PIG-L exhibits a novel metal-independent albeit metal-stimulated activity. Using mutational and biochemical analysis, here we identify Asp-46 and His-140 of the enzyme as being important for catalysis. We show that these mutations neither affect the global conformational of the enzyme nor alter its metal binding affinity. The defect in catalysis, due to the...

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Identifiers

PMID: 23341455
DOI: 10.1074/jbc.M112.427245
PMCID: PMC3597800

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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