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Effective Preparation of Plasmodium vivax Field Isolates for High-Throughput Whole Genome Sequencing

4 Jan 2013

Abstract excerpt

Whole genome sequencing (WGS) of Plasmodium vivax is problematic due to the reliance on clinical isolates which are generally low in parasitaemia and sample volume. Furthermore, clinical isolates contain a significant contaminating background of host DNA which confounds efforts to map short read sequence of the target P. vivax DNA. Here, we discuss a methodology to significantly improve the success of P. vivax...

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PMID: 23308154
DOI: 10.1371/journal.pone.0053160

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