Target-selective homologous recombination cloning for high-throughput generation of monoclonal antibodies from single plasma cells

Abstract excerpt

BACKGROUND: Molecular cloning of functional immunoglobulin genes from single plasma cells is one of the most promising technologies for the rapid development of monoclonal antibody drugs. However, the proper insertion of PCR-amplified immunoglobulin genes into expression vectors remains an obstacle to the high-throughput production of recombinant monoclonal antibodies. RESULTS: We developed a single-step cloning...

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