Article
Heterologous expression of a mutated toxin gene from Bacillus thuringiensis subsp. tenebrionis.
FEMS microbiology letters - 1 Jan 1990
Rhim S L, Jahn N, Schnetter W, Geider K
Abstract excerpt
Using oligonucleotide probes we have isolated a DNA fragment encoding an insecticidal toxin of the coleopteran specific Bacillus thuringiensis subsp. tenebrionis. The gene was altered by site directed mutagenesis at its 5'-end and adapted for general cloning and expression purposes with a linker including a start codon and new restriction sites. The constructs were inserted into several vector plasmids and...
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