Article
Heterologous expression of a mutated toxin gene from Bacillus thuringiensis subsp. tenebrionis.
FEMS microbiology letters - 1 Jan 1990
Rhim S L, Jahn N, Schnetter W, Geider K
Abstract excerpt
Using oligonucleotide probes we have isolated a DNA fragment encoding an insecticidal toxin of the coleopteran specific Bacillus thuringiensis subsp. tenebrionis. The gene was altered by site directed mutagenesis at its 5'-end and adapted for general cloning and expression purposes with a linker...
Topics
- Animals
- Bacillus thuringiensis
- Bacillus thuringiensis Toxins
- Bacterial Proteins
- Bacterial Toxins
- Base Sequence
- Cloning, Molecular
- Coleoptera
- DNA, Bacterial
- Endotoxins
- Gene Expression Regulation, Bacterial
- Genes, Bacterial