Purification and characterization of Bacillus thuringiensis var. tenebrionis insecticidal proteins produced in E. coli.

doi:10.1016/0006-291x(90)92143-n

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Article

Purification and characterization of Bacillus thuringiensis var. tenebrionis insecticidal proteins produced in E. coli.

Biochemical and biophysical research communications - 31 Jul 1990

MacIntosh S C, McPherson S L, Perlak F J, Marrone P G, Fuchs R L

Abstract excerpt

Native and single amino acid variants of the Bacillus thuringiensis var. tenebrionis insecticidal proteins were expressed in Escherichia coli, purified and examined for biological and biochemical properties. A novel, pH dependent, preferential precipitation method was implemented to purify Escher...

Topics

Animals
Bacillus thuringiensis
Bacillus thuringiensis Toxins
Bacterial Proteins
Bacterial Toxins
Coleoptera
Cysteine
Endotoxins
Escherichia coli
Genes, Bacterial
Hemolysin Proteins
Mutation
Recombinant Proteins
Serine

Source

Open in PubMed

Identifiers

PMID: 2116794
DOI: 10.1016/0006-291x(90)92143-n

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2116794-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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