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Purification and characterization of Bacillus thuringiensis var. tenebrionis insecticidal proteins produced in E. coli.

Biochemical and biophysical research communications - 31 Jul 1990

MacIntosh S C, McPherson S L, Perlak F J, Marrone P G, Fuchs R L

Abstract excerpt

Native and single amino acid variants of the Bacillus thuringiensis var. tenebrionis insecticidal proteins were expressed in Escherichia coli, purified and examined for biological and biochemical properties. A novel, pH dependent, preferential precipitation method was implemented to purify Escherichia coli produced Bacillus thuringiensis var. tenebrionis proteins, which are active against Colorado potato beetle...

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PMID: 2116794
DOI: 10.1016/0006-291x(90)92143-n

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