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Article

Allele-specific siRNA silencing for the common keratin 12 founder mutation in Meesmann epithelial corneal dystrophy.

Investigative ophthalmology & visual science - 17 Jan 2013

Allen Edwin H A, Atkinson Sarah D, Liao Haihui, Moore Jonathan E, Pedrioli Deena M Leslie, Smith Frances J D, McLean W H Irwin, Moore C B Tara

Abstract excerpt

PURPOSE: To identify an allele-specific short interfering RNA (siRNA), against the common KRT12 mutation Arg135Thr in Meesmann epithelial corneal dystrophy (MECD) as a personalized approach to treatment. METHODS: siRNAs against the K12 Arg135Thr mutation were evaluated using a dual luciferase reporter gene assay and the most potent and specific siRNAs were further screened by Western blot. Off-target effects on...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Open in PubMed Open full text

Identifiers

PMID: 23233254
DOI: 10.1167/iovs.12-10528
PMCID: PMC3869971

Genes

TLR3
KRT12

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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