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Article

siRNA silencing of the mutant keratin 12 allele in corneal limbal epithelial cells grown from patients with Meesmann's epithelial corneal dystrophy.

Investigative ophthalmology & visual science - 6 May 2014

Courtney David G, Atkinson Sarah D, Allen Edwin H A, Moore Johnny E, Walsh Colum P, Pedrioli Deena M Leslie, MacEwen Caroline J, Pellegrini Graziella, Maurizi Eleonora, Serafini Chiara, Fantacci Monica, Liao Haihui, Irvine Alan D, McLean W H Irwin, Moore C B Tara

Abstract excerpt

PURPOSE: The aim of this study is to further assess our previously reported keratin 12 (K12)-Leu132Pro specific siRNA in silencing the mutant allele in Meesmann's Epithelial Corneal Dystrophy (MECD) in experimental systems more akin to the in vivo situation through simultaneous expression of both wild-type and mutant alleles. METHODS: Using KRT12 exogenous expression constructs transfected into cells, mutant...

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Share to Cell Proliferation
Share to Enzyme-Linked Immunosorbent Assay

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Immunohistochemistry

Share to Limbus Corneae

Share to Mutation, Missense

Share to Real-Time Polymerase Chain Reaction

Share to RNA, Small Interfering

Source

Identifiers

PMID: 24801514
DOI: 10.1167/iovs.13-12957

Genes

KRT12

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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