Article
OsRab11 and OsGAP1 are essential for the vesicle trafficking of the vacuolar H(+)-ATPase OsVHA-a1 under high salinity conditions.
Plant science : an international journal of experimental plant biology - 1 Jan 2013
Son Young Sim, Im Chak Han, Kim Dae Won, Bahk Jeong Dong
Abstract excerpt
To understand the molecular mechanism of the plant vacuolar H(+)-ATPase in endocytic trafficking and adaptation to high salinity, yeast two-hybrid assay, IP-western hybridization, trafficking assay, RT- and qRT-PCR analyses and growth assay were performed here. To confirm the interaction between OsVHA-a1 and OsGAP1, pull-down assay and Co-IP were performed in vitro and in vivo, respectively. qRT-PCR analysis...
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