Article
[Enhanced molecular techniques for the diagnosis of human papillomavirus infections].
Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi - 1 Jan 2000
Ursu Ramona Gabriela, Onofriescu M, Nemescu D, Iancu Luminiţa Smaranda
Abstract excerpt
OBJECTIVES: optimisation of Real Time PCR technique for quantifying oncogenic types 16 and 18 of Human Papilloma Viruses, genotyped through classic PCR, followed by hybridisation. MATERIAL AND METHOD: DNA/ HPV was purified with High Pure PCR Template Preparation kit (ROCHE DIAGNOSTICS), genotyping was performed with Linear Array HPV Genotyping (ROCHE DIAGNOSTICS) and PCR reaction was realized with ABI 9700 Gold...
Topics
- Cervix Uteri
- DNA, Viral
- Female
- Genotype
- Human papillomavirus 16
- Human papillomavirus 18
- Humans
- Papillomavirus Infections
- Polymerase Chain Reaction
- Reproducibility of Results
- Sensitivity and Specificity