Article
A toolkit for bulk PCR-based marker design from next-generation sequence data: application for development of a framework linkage map in bulb onion (Allium cepa L.).
BMC genomics - 19 Nov 2012
Baldwin Samantha, Revanna Roopashree, Thomson Susan, Pither-Joyce Meeghan, Wright Kathryn, Crowhurst Ross, Fiers Mark, Chen Leshi, Macknight Richard, McCallum John A
Abstract excerpt
BACKGROUND: Although modern sequencing technologies permit the ready detection of numerous DNA sequence variants in any organisms, converting such information to PCR-based genetic markers is hampered by a lack of simple, scalable tools. Onion is an example of an under-researched crop with a complex, heterozygous genome where genome-based research has previously been hindered by limited sequence resources and...
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