Article
High-throughput real-time PCR-based genotyping without DNA purification.
BMC research notes - 19 Oct 2012
Fedick Anastasia, Su Jing, Jalas Chaim, Treff Nathan R
Abstract excerpt
BACKGROUND: While improvements in genotyping technology have allowed for increased throughput and reduced time and expense, protocols remain hindered by the slow upstream steps of isolating, purifying, and normalizing DNA. Various methods exist for genotyping samples directly through blood, without having to purify the DNA first. These procedures were designed to be used on smaller throughput systems, however,...
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