Article
Development and application of a SYBR green RT-PCR for first line screening and quantification of porcine sapovirus infection.
BMC veterinary research - 17 Oct 2012
Mauroy Axel, Van der Poel Wim H M, der Honing Renate Hakze-Van, Thys Christine, Thiry Etienne
Abstract excerpt
BACKGROUND: Sapoviruses are single stranded positive sense RNA viruses belonging to the family Caliciviridae. The virus is detected in different species including the human and the porcine species as an enteric pathogen causing asymptomatic to symptomatic enteritis. In this study, we report the development of a rapid real time qRT-PCR based on SYBR Green chemistry for the diagnosis of porcine sapovirus infection...
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