Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Development and validation of a SYBR green real-time PCR for the quantification of porcine circovirus type 2 in serum, buffy coat, feces, and multiple tissues.

Veterinary microbiology - 1 Jan 2009

McIntosh Kathleen A, Tumber Anju, Harding John C S, Krakowka Steven, Ellis John A, Hill Janet E

Abstract excerpt

The emergence of multiple genotypes of PCV2, as demonstrated by phylogenetic analysis of whole genome or capsid sequences, makes it necessary to have quantitative diagnostic assays that perform equally well on all strains. The objectives of this study were to develop and validate a novel real-time polymerase chain reaction (PCR) assay targeting the highly conserved rep gene (ORF1) and investigate the effects of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Benzothiazoles
Share to Circovirus

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Diamines

Share to Germ-Free Life

Share to Lymphoid Tissue

Share to Organic Chemicals

Share to Quinolines

Source

Identifiers

PMID: 18639395
DOI: 10.1016/j.vetmic.2008.06.010

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Development and validation of a SYBR green real-time PCR for the quantification of porcine circovirus type 2 in serum, buffy coat, feces, and multiple tissues.PMID 18639395Read ↗

Development and validation of direct PCR and quantitative PCR assays for the rapid, sensitive, and economical detection of porcine circovirus 3Semantic proximity Read ↗

Detection of PCV2 DNA by SYBR Green I-based quantitative PCRSemantic proximity Read ↗

Development of a Triplex Real-Time PCR Method for the Simultaneous Detection of Porcine Circovirus 2, 3, and 4 in China Between 2023 and 2024Semantic proximity Read ↗

Development and application of a quadruplex real-time PCR method for Torque teno sus virus 1, Porcine circovirus type 2, pseudorabies virus, and porcine parvovirusSemantic proximity Read ↗

PCV2d-2 is the predominant type of PCV2 DNA in pig samples collected in the U.S. during 2014-2016.Semantic proximity Read ↗

Detection of Porcine Circovirus (PCV) Using CRISPR-Cas12a/13a Coupled with Isothermal AmplificationSemantic proximity Read ↗

Porcine circovirus-2 DNA concentration distinguishes wasting from nonwasting pigs and is correlated with lesion distribution, severity, and nucleocapsid staining intensity.Semantic proximity Read ↗

Development of a differential multiplex real-time PCR assay for porcine circovirus type 2 (PCV2) genotypes PCV2a, PCV2b and PCV2d.Semantic proximity Read ↗

Development of a TaqMan-Probe-Based Multiplex Real-Time PCR for the Simultaneous Detection of Porcine Circovirus 2, 3, and 4 in East China from 2020 to 2022Semantic proximity Read ↗

Development and evaluation of a loop-mediated isothermal amplification method for rapid detection and differentiation of two genotypes of porcine circovirus type 2.Semantic proximity Read ↗

Evaluation of a multiplex PCR method for the detection of porcine parvovirus types 1 through 7 using various field samplesSemantic proximity Read ↗

Prevalence and phylogenetic analysis of the current porcine circovirus 2 genotypes after implementation of widespread vaccination programmes in the USA.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.