Multiple Co-Infecting Caliciviruses in Oral Fluid and Enteric Samples of Swine Detected by a Novel RT-qPCR Assay and a 3'RACE-PCR-NGS Method.

doi:10.3390/v17020193

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Multiple Co-Infecting Caliciviruses in Oral Fluid and Enteric Samples of Swine Detected by a Novel RT-qPCR Assay and a 3'RACE-PCR-NGS Method.

Viruses - 30 Jan 2025

László Zoltán, Pankovics Péter, Urbán Péter, Herczeg Róbert, Balka Gyula, Igriczi Barbara, Cságola Attila, Albert Mihály, Tóth Fruzsina, Reuter Gábor, Boros Ákos

Abstract excerpt

Caliciviruses including noro- and sapoviruses of family Caliciviridae are important enteric human and swine pathogens, while others, like valoviruses, are less known. In this study, we developed a detection and typing pipeline for the most prevalent swine enteric caliciviruses-sapovirus GIII (Sw-SaV), norovirus GII (Sw-NoV), and valovirus GI (Sw-VaV). The pipeline integrates triplex RT-qPCR, 3'RACE semi-nested...

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Topics

Animals
Swine
Swine Diseases
Caliciviridae Infections
Caliciviridae
Saliva

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Coinfection

Real-Time Polymerase Chain Reaction

Phylogeny

High-Throughput Nucleotide Sequencing

Feces

Sapovirus

Diarrhea

Genotype

Source

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Identifiers

PMID: 40006947
DOI: 10.3390/v17020193
PMCID: PMC11860220

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