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Maturation induction of human peripheral blood mononuclear cell-derived dendritic cells

2 May 2012

Abstract excerpt

The aim of the present study was to explore an optimal method for maturation induction of dendritic cells (DCs). Human monocyte-derived DCs were induced in the presence of GM-CSF and IL-4. On Day 6, the maturation of DCs was induced with CD40L, LPS, TNF-α and cocktail of cytokines (TNF-α, IL-6, IL-1β and PGE2), respectively, for 24 h. Then, DCs were harvested and subjected to flow cytometry (FCM) for the...

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PMID: 23060936
DOI: 10.3892/etm.2012.565

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