Article
Diversity of hydrolases from hydrothermal vent sediments of the Levante Bay, Vulcano Island (Aeolian archipelago) identified by activity-based metagenomics and biochemical characterization of new esterases and an arabinopyranosidase.
Applied microbiology and biotechnology - 1 Dec 2015
Placido Antonio, Hai Tran, Ferrer Manuel, Chernikova Tatyana N, Distaso Marco, Armstrong Dale, Yakunin Alexander F, Toshchakov Stepan V, Yakimov Michail M, Kublanov Ilya V, Golyshina Olga V, Pesole Graziano, Ceci Luigi R, Golyshin Peter N
Abstract excerpt
A metagenomic fosmid expression library established from environmental DNA (eDNA) from the shallow hot vent sediment sample collected from the Levante Bay, Vulcano Island (Aeolian archipelago) was established in Escherichia coli. Using activity-based screening assays, we have assessed 9600 fosmid clones corresponding to approximately 350 Mbp of the cloned eDNA, for the lipases/esterases/lactamases, haloalkane and...
Topics
- Escherichia coli
- Gene Library
- Genetic Testing
- Genetic Variation
- Geologic Sediments
- Hydrolases
- Hydrothermal Vents
- Islands
- Italy