Article
A comprehensive comparison of RNA-Seq-based transcriptome analysis from reads to differential gene expression and cross-comparison with microarrays: a case study in Saccharomyces cerevisiae.
Nucleic acids research - 1 Nov 2012
Nookaew Intawat, Papini Marta, Pornputtapong Natapol, Scalcinati Gionata, Fagerberg Linn, Uhlén Matthias, Nielsen Jens
Abstract excerpt
RNA-seq, has recently become an attractive method of choice in the studies of transcriptomes, promising several advantages compared with microarrays. In this study, we sought to assess the contribution of the different analytical steps involved in the analysis of RNA-seq data generated with the Illumina platform, and to perform a cross-platform comparison based on the results obtained through Affymetrix...
Topics
- Base Sequence
- Chromosome Mapping
- Data Interpretation, Statistical
- Gene Expression Profiling
- Genome, Fungal
- High-Throughput Nucleotide Sequencing
- INDEL Mutation
- Molecular Sequence Data
- Oligonucleotide Array Sequence Analysis