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Article

Changing the start codon context of the 30K gene of tobacco mosaic virus from "weak" to "strong" does not increase expression.

Virology - 1 Jan 1990

Lehto K, Dawson W O

Abstract excerpt

The translation initiation region of the 30K gene of tobacco mosaic virus (TMV) was modified by in vitro mutagenesis to create more optimal start codon contexts. A complicating factor was that modifications in this region also altered the 3' terminus of the 183K ORF that overlaps the 30K ORF. An insertion of GACUCGA between nucleotides 4901 and 4902 resulted in a purine (G) in position -3 relative to the AUG...

Topics

Base Sequence
Blotting, Western
Codon
Gene Expression Regulation, Viral
Molecular Sequence Data
Mutation
Protein Biosynthesis
RNA, Messenger
RNA, Viral
Tobacco Mosaic Virus
Transcription, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Virus Replication

Source

Identifiers

PMID: 2294639
DOI: 10.1016/0042-6822(90)90065-y

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2294639-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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