Article
Replication, stability, and gene expression of tobacco mosaic virus mutants with a second 30K ORF.
Virology - 1 Mar 1990
Lehto K, Dawson W O
Abstract excerpt
A series of tobacco mosaic virus (TMV)-hybrids containing a second 30K open reading frame (ORF) inserted into different positions of the genome 3' region were constructed. These insertional mutants were used to evaluate the effects of a modified viral genome organization on replication and gene expression. They were evaluated for stability upon systemic infection and subsequent host passage using RNase protection...
Topics
- Base Sequence
- Blotting, Western
- Capsid
- Gene Expression
- Genes, Viral
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Plants, Toxic
- RNA, Viral
- Restriction Mapping
- Nicotiana
