Article
Stress effects caused by the expression of a mutant cellobiohydrolase I and proteasome inhibition in Trichoderma reesei Rut-C30.
New biotechnology - 25 Jan 2013
Kautto Liisa, Grinyer Jasmine, Paulsen Ian, Tetu Sasha, Pillai Aneesh, Pardiwalla Swapneel, Sezerman Ugur, Akcapinar Gunseli Bayaram, Bergquist Peter, Te'o Junior, Nevalainen Helena
Abstract excerpt
Trichoderma reesei Rut-C30 is used widely as an expression host for various gene products. We have explored cellular effects caused by the expression of a mutant form of cellobiohydrolase I (CBHI), the major secreted protein of T. reesei using biochemical and transcriptomic analyses and confocal laser scanning microscopy. The mutated CBHI was tagged fluorescently with Venus to establish the subcellular location...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
