Inaccurate identification of rotavirus genotype G9 as genotype G3 strains due to primer mismatch.

doi:10.1186/1743-422X-9-144

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Article

Inaccurate identification of rotavirus genotype G9 as genotype G3 strains due to primer mismatch.

Virology journal - 3 Aug 2012

Mitui Marcelo Takahiro, Chandrasena Tga Nilmini, Chan Paul Ks, Rajindrajith Shaman, Nelson E Anthony S, Leung Ting Fan, Nishizono Akira, Ahmed Kamruddin

Abstract excerpt

Reverse transcription (RT)-PCR is now the standard method for typing group A rotaviruses (RVA) to monitor the circulating genotypes in a population. Selection of primers that can accurately type the circulating genotypes is crucial in the context of vaccine introduction and correctly interpreting the impact of vaccination on strain distribution. To our knowledge this study is the first report from Asia of...

Topics

Child, Preschool
DNA Primers
Diagnostic Errors
Genotype
Hong Kong
Humans
Infant
Infant, Newborn
Reverse Transcriptase Polymerase Chain Reaction
Rotavirus
Rotavirus Infections
Sri Lanka

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Identifiers

PMID: 22862784
DOI: 10.1186/1743-422X-9-144
PMCID: PMC3422167

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