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Detection and differentiation of avian reoviruses using SYBR-Green I-based two-step real-time reverse transcription PCR with melting curve analysis.

Avian diseases - 1 Jun 2012

Guo Kejun, Dormitorio Teresa, Ou Shan-Cia, Giambrone Joseph

Abstract excerpt

A two-step SYBR-Green I-based real-time PCR with melting curve analysis was developed to detect and differentiate the avian reovirus (ARV) sigmaC gene in field and vaccine ARVs. Three primer sets were used to amplify the sigmaC gene from its 5', center, and 3' regions and analyze the melting point temperatures of nine ARVs. By combining the melting curves of the three ARV sigmaC gene regions, melting curve...

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PMID: 22856196
DOI: 10.1637/9692-022211-Reg.1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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