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Article

High-resolution melting curve FRET-PCR rapidly identifies SARS-CoV-2 mutations.

Journal of medical virology - 1 Sept 2021

Barua Subarna, Hoque Monirul, Kelly Patrick J, Bai Jianfa, Hanzlicek Gregg, Noll Lance, Walz Heather, Johnson Calvin, Kyriakis Constantinos, Wang Chengming

Abstract excerpt

Reverse transcription fluorescence resonance energy transfer-polymerase chain reaction (FRET-PCRs) were designed against the two most common mutations in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) (A23403G in the spike protein; C14408T in the RNA-dependent RNA polymerase). Based on high-resolution melting curve analysis, the reverse transcription (RT) FRET-PCRs identified the mutations in...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34138474
DOI: 10.1002/jmv.27139
PMCID: PMC8426997

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