Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Novel PKCα-mediated phosphorylation site(s) on cofilin and their potential role in terminating histamine release.

Molecular biology of the cell - 1 Sept 2012

Sakuma Megumi, Shirai Yasuhito, Yoshino Ken-ichi, Kuramasu Maho, Nakamura Tomofumi, Yanagita Toshihiko, Mizuno Kensaku, Hide Izumi, Nakata Yoshihiro, Saito Naoaki

Abstract excerpt

Using specific inhibitors, kinase-negative mutants, and small interfering RNA against protein kinase Cα (PKCα) or PKCβI, we find that PKCβI positively regulates degranulation in rat basophilic leukemia-2H3 cells, whereas PKCα negatively regulates degranulation. Mass spectrometric and mutagenic analyses reveal that PKCα phosphorylates cofilin at Ser-23 and/or Ser-24 during degranulation. Overexpression of a...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to 14-3-3 Proteins
Share to Cell Degranulation

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Indoles

Share to Maleimides

Share to Microscopy, Confocal

Share to Polymerization

Share to Protein Kinase C-alpha

Share to Serine

Source

Open in PubMed Open full text

Identifiers

PMID: 22855535
DOI: 10.1091/mbc.E12-01-0053
PMCID: PMC3442417

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Novel PKCα-mediated phosphorylation site(s) on cofilin and their potential role in terminating histamine release.PMID 22855535Read ↗

The actin remodeling protein cofilin is crucial for thymic αβ but not γδ T-cell development.Semantic proximity Read ↗

Unbalancing the Phosphatidylinositol-4,5-bisphosphate–Cofilin Interaction Impairs Cell SteeringSemantic proximity Read ↗

Identification of protein kinase C phosphorylation sites involved in phorbol ester-induced desensitization of the histamine H1 receptor.Semantic proximity Read ↗

A reducing milieu renders cofilin insensitive to phosphatidylinositol 4,5-bisphosphate (PIP2) inhibition.Semantic proximity Read ↗

Phosphorylation of Ser-3 of cofilin regulates its essential function on actin.Semantic proximity Read ↗

Protein kinase C (PKC) delta suppresses keratinocyte proliferation by increasing p21(Cip1) level by a KLF4 transcription factor-dependent mechanism.Semantic proximity Read ↗

Cofilin 1 is revealed as an inhibitor of glucocorticoid receptor by analysis of hormone-resistant cells.Semantic proximity Read ↗

Deletion of the regulatory domain of protein kinase C alpha exposes regions in the hinge and catalytic domains that mediate nuclear targeting.Semantic proximity Read ↗

Sequestration of phosphoinositides by mutated MARCKS effector domain inhibits stimulated Ca(2+) mobilization and degranulation in mast cells.Semantic proximity Read ↗

Role of putative phosphorylation sites in down-regulation of histamine H1 receptors.Semantic proximity Read ↗

Two activities of cofilin, severing and accelerating directional depolymerization of actin filaments, are affected differentially by mutations around the actin-binding helix.Semantic proximity Read ↗

Shear stress induced reorganization of the keratin intermediate filament network requires phosphorylation by protein kinase C zeta.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.