Article
A flow cytometric assay for target binding by NKH1A+ cells using a single laser system.
Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology - 1 Jul 1990
Zanyk M J, Banerjee D, McFarlane D L
Abstract excerpt
A modified method of analysis by flow cytometry has been adapted to the NK/target cell system to monitor conjugate formation within a mixed population. The two-parameter assay is based on the use of two colour fluorescent markers in which an indirect FITC-conjugated NKH1A antibody (green fluorescence) is used to detect NK-specific effector cells and an intracellular strain, hydroethidine (red fluorescence) is...
Topics
- Antibodies, Monoclonal
- Cell Adhesion
- Cell Separation
- Flow Cytometry
- Fluorescent Dyes
- Humans
- Killer Cells, Natural
- Lasers
- Neoplasms
- Phenotype
- Tumor Cells, Cultured
