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Article

Simple and rapid molecular techniques for identification of amylose levels in rice varieties.

International journal of molecular sciences - 1 Jan 2012

Cheng Acga, Ismail Ismanizan, Osman Mohamad, Hashim Habibuddin

Abstract excerpt

The polymorphisms of Waxy (Wx) microsatellite and G-T single-nucleotide polymorphism (SNP) in the Wx gene region were analyzed using simplified techniques in fifteen rice varieties. A rapid and reliable electrophoresis method, MetaPhor agarose gel electrophoresis (MAGE), was effectively employed as an alternative to polyacrylamide gel electrophoresis (PAGE) for separating Wx microsatellite alleles. The amplified...

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PMID: 22754356
DOI: 10.3390/ijms13056156
PMCID: PMC3382826

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