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Article

Homotrimer formation and dissociation of pharaonis halorhodopsin in detergent system.

Biophysical journal - 20 Jun 2012

Tsukamoto Takashi, Sasaki Takanori, Fujimoto Kazuhiro J, Kikukawa Takashi, Kamiya Masakatsu, Aizawa Tomoyasu, Kawano Keiichi, Kamo Naoki, Demura Makoto

Abstract excerpt

Halorhodopsin from NpHR is a light-driven Cl(-) pump that forms a trimeric NpHR-bacterioruberin complex in the native membrane. In the case of NpHR expressed in Escherichia coli cell, NpHR forms a robust homotrimer in a detergent DDM solution. To identify the important residue for the homotrimer formation, we carried out mutation experiments on the aromatic amino acids expected to be located at the molecular...

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Identifiers

PMID: 22735541
DOI: 10.1016/j.bpj.2012.05.008
PMCID: PMC3379016

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