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Article

Specific plate assay for bacterial heparinase.

Applied and environmental microbiology - 1 Nov 1990

Zimmermann J J, Langer R, Cooney C L

Abstract excerpt

A procedure was developed for detecting heparinase activity on heparin agar plates. The method is based on the differential precipitation of heparin and heparinase-generated heparin fragments by protamine sulfate. Heparinase activity is detected by the presence of clear zones against a white background. This method can be used to screen for the expression of recombinant heparinase and to identify Flavobacterium...

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Identifiers

PMID: 2268165
DOI: 10.1128/aem.56.11.3593-3594.1990
PMCID: PMC185029

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