Article
Optimization of factors affecting Agrobacterium-mediated transformation of Micro-Tom tomatoes.
Genetics and molecular research : GMR - 16 Mar 2012
Guo M, Zhang Y L, Meng Z J, Jiang J
Abstract excerpt
Micro-Tom is the smallest known variety of tomatoes. An orthogonal experimental design L(16) (4(5)) was used to optimize Agrobacterium-mediated transformation of cotyledon explants of Lycopersicon esculentum cv. Micro-Tom. Four parameters were investigated to determine their effect on transformation frequency: the concentration of bacterial suspension, time of dip in bacterial suspension, co-cultivation...
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