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Article

An efficient mannose selection protocol for tomato that has no adverse effect on the ploidy level of transgenic plants.

Plant cell reports - 1 Oct 2004

Sigareva Marina, Spivey Rody, Willits Michael G, Kramer Catherine M, Chang Yin-Fu

Abstract excerpt

A protocol for Agrobacterium-mediated transformation with mannose selection was developed for cotyledon petiole, hypocotyl and leaf explants of tomato (Lycopersicon esculentum L. Mill). More than 400 transgenic plants from three tomato varieties were selected with 1% mannose in combination with 0.1-0.5% glucose. Average transformation frequencies ranged from 2.0 to 15.5% depending on the construct, genotype and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 15197480
DOI: 10.1007/s00299-004-0809-8

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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