Article
[Mutagenesis of vitamin B2 producer by using protoplasts].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Aug 1990
Li Q, Zhu B, Li L, An H, Wang J
Abstract excerpt
Studies on preparation, regeneration and ultraviolet-mutagenesis of protoplasts of vitamin B2 producer Eremothecium aohbyii were reported. By using a complex enzyme system (0.5% snial digestase + 0.5% cellulase), a great number of protoplasts were obtained. Ultraviolet-mutagenesising positive mutation ratio is 14.29%. The HPLC analysis indicated that a lot of mutants were screened by using ultraviolet-mutagenesis...
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