Article
[Breeding of high-yield strain of taxol by mutagensis of protoplast and primary discussion of genetic differences between mutants and their parent strain].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Jun 2005
Zhao Kai, Ping Wen-xiang, Ma Xi, Liu Jun, Zhou Dong-po
Abstract excerpt
The breeding of high-yield strain of taxol was performed by protoplast mutagenesis of strain NCEU-1 using ultraviolet radiation and combined treatment of UV and LiCl. The mutants UV40-19 and UL50-6 were obtained, which raised the taxol yield from 314.07 microg/L to 376.38 microg/L and 392.63 microg/L respectively. Genetic differences between the mutants UV40-19, UL50-6 and their parent strain were primarily...
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