Article
Laser mutagenesis of protoplasts of Penicillium sp. PT95 for the enhancement of carotenoid yield.
Journal of applied microbiology - 1 Nov 2008
Han J R, Wang Q
Abstract excerpt
AIMS: To isolate the protoplasts from Penicillium sp. PT95 and carry out laser mutagenesis to attain high-yield mutant strain for carotenoid production. METHODS AND RESULTS: The mycelial pellets of PT95 strain were digested with the lytic enzyme for 3 h in order to attain protoplasts. The prepared protoplasts were irradiated using helium neon (He-Ne) laser. Among all regenerated colonies isolated from irradiated...
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