Article
[Study on breeding up high-yield strain of taxol by protoplast mutagensis].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Sept 2005
Zhao Kai, Zhou Dong-Po, Ping Wen-Xiang, Liu Jun, Ma Xi, Jin Tao
Abstract excerpt
In order to obtain resistant mutants to nystatin, ultraviolet radiation and LiCl were used to mutagenize the protoplasts of taxol-producing fungi NCEU-1, and four positive mutants with high yield of taxol were screened out on nystatin flat. After further screening experiments on fermentation, a mutant strain--UL04-5 which was able to produce taxol with high yield and could be stably passed on in genetics was...
Topics
- Ascomycota
- Fermentation
- Genetic Variation
- Mutagenesis
- Nystatin
- Paclitaxel
- Protoplasts