Article
Baculovirus expression and antigenic characterization of classical swine fever virus E2 proteins.
Transboundary and emerging diseases - 1 Apr 2013
Luo L, Nishi K, Macleod E, Sabara M I, Lin M, Handel K, Pasick J
Abstract excerpt
Genes encoding a major structural glycoprotein, E2, of classical swine fever viruses (CSFV) Brescia (subgroup 1.2), Paderborn (subgroup 2.1) and Kanagawa (subgroup 3.4) were constructed by removing the transmembrane domain and adding a C-terminal 6 histidine (His) tag. All the E2 constructs were efficiently expressed in a baculovirus system as 53-kDa glycosylated proteins that were identified in Western blots by...
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