Article
Forensic STR analysis using massive parallel sequencing.
Forensic science international. Genetics - 1 Dec 2012
Van Neste Christophe, Van Nieuwerburgh Filip, Van Hoofstat David, Deforce Dieter
Abstract excerpt
We explore the applicability of second generation sequencing (SGS) to sequence multiplexed forensic STR amplicons, both in a single contributor sample as in multiple-person mixtures with different ratios. We compare the results of a commercial STR profiling kit (Applied Biosystems AmpFlSTR(®) Profiler Plus(®)), analyzed both with capillary electrophoresis and with Roche GS FLX sequencing. An easy to use...
Topics
- Alleles
- DNA Fingerprinting
- Electrophoresis, Capillary
- High-Throughput Nucleotide Sequencing
- Humans
- Microsatellite Repeats
- Multiplex Polymerase Chain Reaction
- Sequence Analysis, DNA