Article
High-throughput sequencing of core STR loci for forensic genetic investigations using the Roche Genome Sequencer FLX platform.
BioTechniques - 1 Aug 2011
Fordyce Sarah L, Ávila-Arcos Maria C, Rockenbauer Eszter, Børsting Claus, Frank-Hansen Rune, Petersen Frederik Torp, Willerslev Eske, Hansen Anders J, Morling Niels, Gilbert M Thomas P
Abstract excerpt
The analysis and profiling of short tandem repeat (STR) loci is routinely used in forensic genetics. Current methods to investigate STR loci, including PCR-based standard fragment analyses and capillary electrophoresis, only provide amplicon lengths that are used to estimate the number of STR repeat units. These methods do not allow for the full resolution of STR base composition that sequencing approaches could...
Topics
- Algorithms
- Alleles
- Computational Biology
- DNA
- Electrophoresis, Capillary
- Forensic Genetics
- Genetic Variation
- High-Throughput Screening Assays
- Humans
- Microsatellite Repeats
- Polymerase Chain Reaction
- Sequence Analysis, DNA