Article
Isolation of polyamine transport-deficient mutants of Escherichia coli and cloning of the genes for polyamine transport proteins.
The Journal of biological chemistry - 5 Dec 1990
Kashiwagi K, Hosokawa N, Furuchi T, Kobayashi H, Sasakawa C, Yoshikawa M, Igarashi K
Abstract excerpt
Escherichia coli KK313, which was deficient in spermidine transport, was isolated by treatment of E. coli MA261 with N-methyl-N'-nitro-N-nitrosoguanidine. E. coli NH1596, which was deficient in spermidine transport and has a 90% decreased putrescine transport activity, was obtained by a second treatment of E. coli KK313 with the same mutagen. Genes for polyamine transport systems were isolated by transforming E....
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