Article
Isolation of polyamine transport-deficient mutants of Escherichia coli and cloning of the genes for polyamine transport proteins.
The Journal of biological chemistry - 5 Dec 1990
Kashiwagi K, Hosokawa N, Furuchi T, Kobayashi H, Sasakawa C, Yoshikawa M, Igarashi K
Abstract excerpt
Escherichia coli KK313, which was deficient in spermidine transport, was isolated by treatment of E. coli MA261 with N-methyl-N'-nitro-N-nitrosoguanidine. E. coli NH1596, which was deficient in spermidine transport and has a 90% decreased putrescine transport activity, was obtained by a second tr...
Topics
- Bacterial Proteins
- Biological Transport
- Carrier Proteins
- Cloning, Molecular
- Escherichia coli
- Genes, Bacterial
- Kinetics
- Methylnitronitrosoguanidine
- Mutation
- Polyamines